Vol. 39 No. 4 (2022): August
Original Article

Determination of reference value for lymphocyte subsets CD3+, CD4+ y CD8+ and CD4+/CD8+ ratio in the healthy Chilean population

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  • Hildegard Kranen,
  • Matias Ortuzar Galaz,
  • Luisa Montecinos Perret,
  • Marcelo Ortega-Riveros,
  • William Acevedo Muñoz
Hildegard Kranen
Hospital de Enfermedades Infecciosas Dr. Lucio Córdova.
Bio
Matias Ortuzar Galaz
Hospital de Enfermedades Infecciosas Dr. Lucio Córdova.
Bio
Luisa Montecinos Perret
Hospital de Enfermedades Infecciosas Dr. Lucio Córdova.
Bio
Marcelo Ortega-Riveros
Universidad del País Vasco / Euskal Herriko Unibertsitatea.
Bio
William Acevedo Muñoz
Hospital de Enfermedades Infecciosas Dr. Lucio Córdova.
Bio

Published 2022-10-04

Keywords

How to Cite

1.
Kranen H, Ortuzar Galaz M, Montecinos Perret L, Ortega-Riveros M, Acevedo Muñoz W. Determination of reference value for lymphocyte subsets CD3+, CD4+ y CD8+ and CD4+/CD8+ ratio in the healthy Chilean population. Rev. Chilena. Infectol. [Internet]. 2022 Oct. 4 [cited 2025 Nov. 28];39(4). Available from: https://www.revinf.cl/index.php/revinf/article/view/1100

Copyright (c) 2022 Hildegard Kranen, Matias Ortuzar Galaz, Luisa Montecinos Perret, Marcelo Ortega-Riveros, William Acevedo Muñoz

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Abstract

Background: Several studies have documented the influence of ethnic factors on the distribution of lymphocyte subpopulations; however, the reference intervals used in Chile were obtained from a study carried out in the Netherlands in 1997. Aim: To determine the reference interval for CD3+, CD4+ and CD8+ lymphocyte subpopulations, as well as the CD4+/CD8+ index in the Chilean population. Methods: A total of 200 whole blood samples obtained from healthy adult men and women were analyzed using the method established by CLSI standardized in protocol EP28-A3c from the pre-analytical stage onwards. Results: The reference ranges for CD3+, CD4+ and CD8+ were 54.7- 81.6% (789-2732 cells/µL), 28.1-57.7% (447-1703 cells/µL) and 15.1-38.8% (226-996 cells/µL), respectively. The CD4+/CD8+ index was 0.84-3.77. Discussion: The reference values for lymphocyte subpopulations in the healthy Chilean population are different from those currently used in Chile. These observations show local data that could have implications for the treatment of HIV infection and the reference ranges found in this study could be used to understand the local situation of some patients. Conclusions: Others studies must be done to confirm these observations due to lack of previous data and because this is the first study in Chilean population.

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