Vol. 39 No. 2 (2022): ABRIL
Laboratory and Infectious Diseases

Colistin resistance in carbapenem-resistant Klebsiella pneumoniae isolates from a pediatric hospital from Corrientes, Argentina

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  • Juan Leandro Pellegrini,
  • Clarisa Aguirre,
  • Susana Marina Soto,
  • Laura Marcela Ramona Lovera,
  • Liliana Silvina Lösch,
  • José Alejandro Di Conza,
  • Luis Antonio Merino
Juan Leandro Pellegrini
Instituto de Medicina Regional- Universidad Nacional del Nordeste- Argentina
Clarisa Aguirre
Hospital Pediátrico “Juan Pablo II”, Corrientes
Susana Marina Soto
Laboratorio Central de Redes y Programas, Corrientes
Laura Marcela Ramona Lovera
Hospital Pediátrico “Juan Pablo II”, Corrientes
Liliana Silvina Lösch
Instituto de Medicina Regional, Universidad Nacional del Nordeste, Chaco
José Alejandro Di Conza
Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires
Luis Antonio Merino
Instituto de Medicina Regional, Universidad Nacional del Nordeste, Chaco

Published 2022-06-08

Keywords

How to Cite

1.
Pellegrini JL, Aguirre C, Soto SM, Lovera LMR, Lösch LS, Di Conza JA, Merino LA. Colistin resistance in carbapenem-resistant Klebsiella pneumoniae isolates from a pediatric hospital from Corrientes, Argentina. Rev. Chilena. Infectol. [Internet]. 2022 Jun. 8 [cited 2026 Jan. 14];39(2). Available from: https://www.revinf.cl/index.php/revinf/article/view/1205

Copyright (c) 2022 Juan Leandro Pellegrini, Clarisa Aguirre, Susana Marina Soto, Laura Marcela Ramona Lovera, Liliana Silvina Lösch, José Alejandro Di Conza, Luis Antonio Merino

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background: There is an increase of carbapenem-resistant Klebsiella pneumoniae (CRKP) infections in the pediatric population and epidemiological data are limited. Aim: To calculate the frequency of CRKP in pediatric patients, to determine the in vitro activity of colistin and to detect the presence of mcr-1 gene in said isolates. Methods: 220 isolates of K. pneumoniae were studied in a pediatric hospital between January 2018 and December 2019. Antimicrobial susceptibility was determined by microdilution in broth according to guidelines of CLSI and EUCAST. The genes blaKPC, blaNDM, blaIMP, blaVIM, blaOXA-48 and mcr-1 were detected by polymerase chain reaction (PCR). Results: 9.5% (n: 21) of the isolates were characterized as CRKP, where was observed a resistance to colistin of 47.6% (10/21) with values of MIC50 of 2 μg/mL and MIC90 of ≥4 μg/mL. In 100% of CRKP strains the blaKPC gene was detected and the mcr-1 gene was not found. The resistance profile to other antimicrobials was as follow: gentamicin 100% (n: 21), trimethoprim/sulfamethoxazole 100% (n: 21), ciprofloxacin 100% (n: 21), amikacin 19% (n: 4). All of the isolates were sensitive to ceftazidime/avibactam and tigecycline. Conclusion: This study demonstrates a significant value of resistance to colistin in pediatric patients compared to other last line antimicrobial such as ceftazidime/avibactam and tigecycline.

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